CDIO / Cardio Diagnostics Holdings, Inc. - SEC Filings, Annual Report, Proxy Statement

Cardio Diagnostics Holdings, Inc.

Basic Stats
LEI 529900E3UWQ6LHYRCK14
CIK 1870144
SEC Filings
All companies that sell securities in the United States must register with the Securities and Exchange Commission (SEC) and file reports on a regular basis. These reports include company annual reports (10K, 10Q), news updates (8K), investor presentations (found in 8Ks), insider trades (form 4), ownership reports (13D, and 13G), and reports related to the specific securities sold, such as registration statements and prospectus. This page shows recent SEC filings related to Cardio Diagnostics Holdings, Inc.
SEC Filings (Chronological Order)
This page provides a complete, chronological list of SEC Filings, excluding ownership filings which we provide elsewhere.
September 5, 2025 POS AM

As filed with the U.S. Securities and Exchange Commission on September 5, 2025.

As filed with the U.S. Securities and Exchange Commission on September 5, 2025. Registration No. 333-268759 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 POST-EFFECTIVE AMENDMENT NO. 3 TO FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 6770 87-0925574 (State

September 4, 2025 DEF 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☒ Definitive Proxy State

September 4, 2025 DEFA14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Definitive Proxy State

August 27, 2025 ARS

CARDIO DIAGNOSTICS HOLDINGS, INC. 2024 ANNUAL REPORT UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal ye

CARDIO DIAGNOSTICS HOLDINGS, INC. 2024 ANNUAL REPORT UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2024 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from t

August 14, 2025 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended June 30, 2025 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Cardio Diagno

August 13, 2025 PRE 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☒ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Definitive Proxy State

May 28, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 28, 2025 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HO

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 28, 2025 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Comm

May 16, 2025 CORRESP

May 16, 2025

Correspondence Cardio Diagnostics Holdings Inc 311 W Superior St, Ste 444 Chicago, IL 60654 (855) 226 9991 info@cardiodiagnosticsinc.

May 15, 2025 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended March 31, 2025 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Cardio Diagn

May 13, 2025 EX-3.1

Certificate of Amendment to the Third Amended and Restated Certificate of Incorporation of Cardio Diagnostics Holdings, Inc. dated May 12, 2025

Exhibit 3.1 CERTIFICATE OF AMENDMENT TO THE THIRD AMENDED AND RESTATED CERTIFICATE OF INCORPORATION OF CARDIO DIAGNOSTICS HOLDINGS, INC. Cardio Diagnostics Holdings, Inc. (the “Corporation”), a corporation organized and existing under and by virtue of the General Corporation Law of the State of Delaware (the “DGCL”), does hereby certify: FIRST: Effective upon the filing of this amendment (the “Eff

May 13, 2025 EX-99.1

CARDIO DIAGNOSTICS HoldingS, INC. ANNOUNCES 1-for-30 reverse stock split

Exhibit 99.1 CARDIO DIAGNOSTICS HoldingS, INC. ANNOUNCES 1-for-30 reverse stock split CHICAGO, IL − (Business Wire) – May 8, 2025 – Cardio Diagnostics Holdings, Inc. (“Cardio” or the “Company”) (NASDAQ: CDIO), today announced a 1-for-30 reverse stock split of its common stock effective with the market opening on May 13, 2025. The reverse stock split will take effect immediately after the close of

May 13, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 12, 2025 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HO

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 12, 2025 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Comm

May 7, 2025 CORRESP

Cardio Diagnostics Holdings Inc. 311 W Superior St, Ste 444 Chicago, IL 60654 (855) 226 9991

Correspondence Cardio Diagnostics Holdings Inc. 311 W Superior St, Ste 444 Chicago, IL 60654 (855) 226 9991 May 7, 2025 VIA EDGAR Division of Corporation Finance Office of Life Sciences United States Securities and Exchange Commission Attention: Bonnie Baynes, Angela Connell Re: Cardio Diagnostics Holdings, Inc. Form 10-K for the Fiscal Year Ended December 31, 2024 File No. 001-41097 Set forth bel

April 8, 2025 EX-FILING FEES

Filing Fee Table

Exhibit 107 Calculation of Filing Fee Tables Form S-8 (Form Type) Cardio Diagnostics Holdings, Inc.

April 8, 2025 S-8

As filed with the Securities and Exchange Commission on April 8, 2025

As filed with the Securities and Exchange Commission on April 8, 2025 Registration No.

March 25, 2025 424B3

CARDIO DIAGNOSTICS HOLDINGS, INC. Up to 11,783,256 Shares of Common Stock Up to 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Up to 236,686 Warrants to Purchase Common Stock

Filed pursuant to Rule 424(b)(3) Registration No. 333-268759 PROSPECTUS SUPPLEMENT NO. 1 (to prospectus dated September 5, 2024) CARDIO DIAGNOSTICS HOLDINGS, INC. Up to 11,783,256 Shares of Common Stock Up to 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Up to 236,686 Warrants to Purchase Common Stock This prospectus supplement No. 1 (the “Prospectus Supplement”) updates, ame

March 25, 2025 424B3

CARDIO DIAGNOSTICS HOLDINGS, INC. Up to 1,235,939 Shares of Common Stock

Filed pursuant to Rule 424(b)(3) Registration No. 333-283419 PROSPECTUS SUPPLEMENT NO. 1 (to prospectus dated December 3, 2024) CARDIO DIAGNOSTICS HOLDINGS, INC. Up to 1,235,939 Shares of Common Stock This prospectus supplement No. 1 (the “Prospectus Supplement”) updates, amends and supplements the prospectus dated December 3, 2024 (the “Prospectus”), which forms a part of our Registration Stateme

March 20, 2025 10-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2024 ☐ TRANSITION REPORT PURSUA

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2024 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File No. 001-41097 CARDIO DIAGNOSTICS HO

March 20, 2025 EX-19.1

Securities Insider Trading Policy

Exhibit 19.1 CARDIO DIAGNOSTICS HOLDINGS INC. SECURITIES TRADING POLICY I. Introduction Federal and state securities laws make it illegal for anyone to trade in a company’s securities while in possession of material, nonpublic information relating to that company. This conduct is referred to as “insider trading” and may result in civil or criminal penalties. The purpose of this Securities Trading

March 20, 2025 EX-21.1

List of Subsidiaries

EXHIBIT 21.1 Subsidiaries of Cardio Diagnostics Holdings, Inc. Cardio Diagnostics, Inc., a Delaware corporation

February 21, 2025 EX-99.1

COMPANY UPDATE Revolutionizing Cardiovascular Medicine With Epigenetics and AI

Exhibit 99.1 COMPANY UPDATE Revolutionizing Cardiovascular Medicine With Epigenetics and AI Certain statements and information included in this presentation constitute "forward - looking statements" within the meaning of t he Private Securities Litigation Act of 1995. When used in this presentation, the words or phrases “will”, "will likely result," "expected to," "will continue," " ant icipated,"

February 21, 2025 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 February 21, 2025 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTI

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 February 21, 2025 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation)

February 14, 2025 424B5

Cardio Diagnostics Holdings, Inc. Up to $9,476,508 Common Stock

Filed pursuant to Rule 424(b)(5) Registration No. 333-284775 PROSPECTUS SUPPLEMENT (to prospectus dated February 14, 2025) Cardio Diagnostics Holdings, Inc. Up to $9,476,508 Common Stock We have entered into an at the market offering agreement (the “Sales Agreement”) with Craig-Hallum Capital Group LLC (the “Sales Agent”), relating to the sale of the shares of our common stock offered by this pros

February 13, 2025 CORRESP

Cardio Diagnostics Holdings, Inc. 311 West Superior Street, Suite 444 Chicago, IL 60654 February 13, 2025

Correspondence Cardio Diagnostics Holdings, Inc. 311 West Superior Street, Suite 444 Chicago, IL 60654 February 13, 2025 VIA EDGAR Division of Corporation Finance Office of Life Sciences U.S. Securities & Exchange Commission 100 F Street, NE Washington, D.C. 20549 Attn: Tamika N. Sheppard Re: Cardio Diagnostics Holdings, Inc. Registration Statement on Form S-3 File No. 333-284775 Acceleration Requ

February 7, 2025 EX-FILING FEES

Filing Fee Table

Exhibit 107 Calculation of Filing Fee Tables Form S-3 (Form Type) Cardio Diagnostics Holdings, Inc.

February 7, 2025 S-3

As filed with the Securities and Exchange Commission on February 7, 2025

As filed with the Securities and Exchange Commission on February 7, 2025 Registration No.

December 4, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 December 4, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTIC

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 December 4, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (

December 3, 2024 424B3

CARDIO DIAGNOSTICS HOLDINGS, INC. Up to 1,235,939 Shares of Common Stock

Filed Pursuant to Rule 424(b)(3) Registration No. 333-283419 PROSPECTUS CARDIO DIAGNOSTICS HOLDINGS, INC. Up to 1,235,939 Shares of Common Stock This prospectus relates to the resale from time to time by the selling stockholders (which term, as used in this prospectus, includes pledgees, donees, transferees or other successors-in-interest) of up to an aggregate of 1,235,939 shares of common stock,

December 2, 2024 CORRESP

Cardio Diagnostics Holdings, Inc. 311 West Superior Street, Suite 444 Chicago, IL 60654 December 2, 2024

Correspondence Cardio Diagnostics Holdings, Inc. 311 West Superior Street, Suite 444 Chicago, IL 60654 December 2, 2024 VIA EDGAR Division of Corporation Finance Office of Life Sciences U.S. Securities & Exchange Commission 100 F Street, NE Washington, D.C. 20549 Attn: Tamika N. Sheppard Re: Cardio Diagnostics Holdings, Inc. Registration Statement on Form S-1 File No. 333-283419 Acceleration Reque

November 22, 2024 EX-FILING FEES

Filing Fee Table

Exhibit 107 Calculation of Filing Fee Tables Form S-1 (Form Type) Cardio Diagnostics Holdings, Inc.

November 22, 2024 S-1

As filed with the U.S. Securities and Exchange Commission on November 22, 2024.

As filed with the U.S. Securities and Exchange Commission on November 22, 2024. Registration No. 333- UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 6770 87-0925574 (State or other jurisdiction of incorporation o

November 18, 2024 8-K

Financial Statements and Exhibits, Submission of Matters to a Vote of Security Holders

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 November 15, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation)

November 13, 2024 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended September 30, 2024 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Cardio D

October 4, 2024 DEF 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☒ Definitive Proxy State

October 4, 2024 DEFA14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Definitive Proxy State

September 25, 2024 ARS

CARDIO DIAGNOSTICS HOLDINGS, INC. 2023 ANNUAL REPORT UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal ye

CARDIO DIAGNOSTICS HOLDINGS, INC. 2023 ANNUAL REPORT UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2023 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from t

September 23, 2024 PRE 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☒ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Definitive Proxy State

August 30, 2024 POS AM

As filed with the U.S. Securities and Exchange Commission on August 30, 2024.

As filed with the U.S. Securities and Exchange Commission on August 30, 2024. Registration No. 333-268759 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 POST-EFFECTIVE AMENDMENT NO. 2 TO FORM S-1 POS AM REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 6770 87-0925574 (S

August 12, 2024 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended June 30, 2024 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Cardio Diagno

June 7, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 June 7, 2024 (June 3, 2024) Date of Report (Date of earliest event reported) CARDIO

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 June 7, 2024 (June 3, 2024) Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incor

May 15, 2024 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended March 31, 2024 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Cardio Diagn

April 26, 2024 S-8

As filed with the Securities and Exchange Commission on April 26, 2024

As filed with the Securities and Exchange Commission on April 26, 2024 Registration No.

April 26, 2024 EX-FILING FEES

Filing Fee Table

Exhibit 107 Calculation of Filing Fee Tables Form S-8 (Form Type) Cardio Diagnostics Holdings, Inc.

April 2, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 April 2, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS H

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 April 2, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Com

April 2, 2024 EX-99.1

EX-99.1

COMPANY UPDATE Revolutionizing Cardiovascular Medicine With Epigenetics and AI M A R C H 2 0 2 4Certain statements and information included in this presentation constitute "forward-looking statements" within the meaning of the Private Securities Litigation Act of 1995.

April 2, 2024 424B3

CARDIO DIAGNOSTICS HOLDINGS, INC. Primary Offering of 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Secondary Offering of 11,883,256 Shares of Common Stock 236,686 Warrants to Purchase Shares of Common Stock

Filed pursuant to Rule 424(b)(3) Registration No. 333-268759 PROSPECTUS SUPPLEMENT NO. 2 (to prospectus dated April 28, 2023) CARDIO DIAGNOSTICS HOLDINGS, INC. Primary Offering of 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Secondary Offering of 11,883,256 Shares of Common Stock 236,686 Warrants to Purchase Shares of Common Stock This prospectus supplement updates, amends a

April 2, 2024 EX-99.1

COMPANY UPDATE Revolutionizing Cardiovascular Medicine With Epigenetics and AI MARCH 2024

Exhibit 99.1 COMPANY UPDATE Revolutionizing Cardiovascular Medicine With Epigenetics and AI MARCH 2024 Certain statements and information included in this presentation constitute "forward - looking statements" within the meaning of t he Private Securities Litigation Act of 1995. When used in this presentation, the words or phrases “will”, "will likely result," "expected to," "will continue," " ant

April 1, 2024 EX-21.1

List of Subsidiaries

EXHIBIT 21.1 Subsidiaries of Cardio Diagnostics Holdings, Inc. Cardio Diagnostics, Inc., a Delaware corporation

April 1, 2024 EX-10.4

Cardio Diagnostics Holdings, Inc. 2022 Equity Incentive Plan and related forms of agreements

Exhibit 10.4 CARDIO DIAGNOSTICS HOLDINGS INC. 2022 EQUITY INCENTIVE PLAN Table of Contents Page SECTION 1 Establishment and Purpose. 5 (a) Purpose. 5 (b) Adoption and Term. 5 SECTION 2 Definitions. 5 SECTION 3 Administration. 9 (a) Committee of the Board of Directors. 10 (b) Authority. 10 (c) Exchange Program. 10 (d) Delegation by the Committee. 10 (e) Indemnification. 11 SECTION 4 Eligibility and

April 1, 2024 EX-4.5

Description of Securities

EXHIBIT 4.5 DESCRIPTION OF THE REGISTRANT’S SECURITIES REGISTERED PURSUANT TO SECTION 12 OF THE SECURITIES EXCHANGE ACT OF 1934 The following is a description of the capital stock of Cardio Diagnostics Holdings, Inc. (“Cardio,” the “Company,” “we,” “us,” and “our”) and certain provisions of our third amended and restated certificate of incorporation (the “certificate of incorporation”), our bylaws

April 1, 2024 EX-97.1

Cardio Diagnostics Holdings, Inc. “Clawback” Policy

EXHIBIT 97.1 Cardio Diagnostics Holdings, Inc. Compensation Recovery Policy 1. Introduction The Board of Directors (the “Board”) of Cardio Diagnostics Holdings, Inc., a corporation organized under the laws of Delaware (the “Company”), has adopted this policy (this “Policy”), which provides for the recovery of erroneously awarded Incentive-based Compensation (as defined below) from current and form

April 1, 2024 10-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2023 ☐ TRANSITION REPORT PURSUA

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2023 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File No. 001-41097 CARDIO DIAGNOSTICS HO

February 2, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 February 2, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTIC

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 February 2, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (

February 2, 2024 EX-4.1

Form of Private Placement Warrant

Exhibit 4.1 FORM OF WARRANT THIS WARRANT AND THE SHARES OF COMMON STOCK ISSUABLE UPON EXERCISE OF THIS WARRANT HAVE NOT BEEN REGISTERED UNDER THE SECURITIES ACT OF 1933, AS AMENDED (THE “ACT”), OR THE SECURITIES LAWS OF ANY STATE AND MAY NOT BE OFFERED, SOLD, TRANSFERRED, PLEDGED, HYPOTHECATED OR OTHERWISE DISPOSED OF EXCEPT PURSUANT TO (I) AN EFFECTIVE REGISTRATION STATEMENT UNDER THE ACT AND ANY

February 1, 2024 424B5

Cardio Diagnostics Holdings, Inc. Up to $17,000,000 Common Stock

Filed pursuant to Rule 424(b)(5) Registration No. 333-276725 PROSPECTUS SUPPLEMENT (to prospectus dated February 1, 2024) Cardio Diagnostics Holdings, Inc. Up to $17,000,000 Common Stock We have entered into an at the market offering agreement (the “Sales Agreement”) with Craig-Hallum Capital Group LLC (the “Sales Agent”), relating to the sale of the shares of our common stock offered by this pros

February 1, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 January 26, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTIC

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 January 26, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (

January 30, 2024 CORRESP

Cardio Diagnostics Holdings, Inc. 311 W. Superior Street, Suite 444 Chicago, IL 60654 January 30, 2024

Correspondence Cardio Diagnostics Holdings, Inc. 311 W. Superior Street, Suite 444 Chicago, IL 60654 January 30, 2024 VIA EDGAR Division of Corporation Finance Office of Life Sciences U.S. Securities & Exchange Commission 100 F Street, NE Washington, D.C. 20549 Attn: Daniel Crawford Re: Cardio Diagnostics Holdings, Inc. Registration Statement on Form S-3 File No. 333-276725 Acceleration Request Re

January 29, 2024 DEL AM

Cardio Diagnostics Holdings, Inc. 411 W. Superior Street, Suite 444 Chicago, IL 60654 January 29, 2024

Cardio Diagnostics Holdings, Inc. 411 W. Superior Street, Suite 444 Chicago, IL 60654 January 29, 2024 VIA EDGAR Division of Corporation Finance Office of Life Sciences U.S. Securities & Exchange Commission 100 F Street, NE Washington, D.C. 20549 Re: Cardio Diagnostics Holdings, Inc. Registration Statement on Form S-3 File No. 333-276725 Ladies and Gentlemen: Please accept this letter as a delayin

January 26, 2024 EX-1.2

At the Market Offering Agreement, dated January 26, 2024, between Cardio Diagnostics Holdings, Inc. and Craig-Hallum Capital Group, LLC

Exhibit 1.2 AT THE MARKET OFFERING AGREEMENT January 26, 2024 Craig-Hallum Capital Group LLC 222 South 9th Street, Suite 350 Minneapolis, MN 55402 Ladies and Gentlemen: Cardio Diagnostics Holdings, Inc., a corporation organized under the laws of Delaware (the “Company”), confirms its agreement (this “Agreement”) with Craig-Hallum Capital Group LLC (the “Manager”) as follows: 1. Definitions. The te

January 26, 2024 S-3

As filed with the Securities and Exchange Commission on January 26, 2024

As filed with the Securities and Exchange Commission on January 26, 2024 Registration No.

January 26, 2024 EX-FILING FEES

Filing Fee Table

Exhibit 107 Calculation of Filing Fee Tables Form S-3 (Form Type) Cardio Diagnostics Holdings, Inc.

January 4, 2024 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 January 4, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 January 4, 2024 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (C

December 19, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 December 18, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTI

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 December 18, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation)

December 6, 2023 8-K

Financial Statements and Exhibits, Other Events

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 December 6, 2023 (December 5, 2023) Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction

December 6, 2023 EX-99.1

Cardio Diagnostics Holdings, Inc. Regains Nasdaq Minimum Bid Price Requirement

Exhibit 99.1 Cardio Diagnostics Holdings, Inc. Regains Nasdaq Minimum Bid Price Requirement CHICAGO, December 6, 2023 - Cardio Diagnostics Holdings, Inc. (Nasdaq: CDIO) (“Cardio Diagnostics” or the “Company”), a leader in artificial intelligence-driven precision cardiovascular medicine tests, today announced that on December 5, 2023, it received a notification letter (the “Notification Letter”) fr

November 22, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 November 22, 2023 (November 20, 2023) Date of Report (Date of earliest event report

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 November 22, 2023 (November 20, 2023) Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdictio

November 22, 2023 DEFA14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (RULE 14a-101) INFORMATION REQUIRED IN PROXY STATEMENT SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Ame

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A (RULE 14a-101) INFORMATION REQUIRED IN PROXY STATEMENT SCHEDULE 14A INFORMATION Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 (Amendment No. ) Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confide

November 13, 2023 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended September 30, 2023 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Cardio D

October 27, 2023 DEF 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☒ Definitive Proxy State

October 12, 2023 PRE 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☒ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Definitive Proxy State

September 25, 2023 8-K

Notice of Delisting or Failure to Satisfy a Continued Listing Rule or Standard; Transfer of Listing, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 25, 2023 (September 21, 2023) Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdict

September 14, 2023 EX-10.1

Letter Agreement dated September 13, 2023 amending the Securities Purchase Agreement dated March 8, 2023

Exhibit 10.1 September 13, 2023 Cardio Diagnostic Holdings, Inc. 400 North Aberdeen Street, Suite 900 Chicago, IL 60642 Ladies and Gentlemen: Reference is made to that certain Securities Purchase Agreement dated March 8, 2023, as amended (the “SPA”), by and between YA II PN, Ltd. (“Yorkville”) and Cardio Diagnostics Holdings, Inc. (the “Company”). Capitalized terms used herein and not otherwise de

September 14, 2023 8-K/A

Entry into a Material Definitive Agreement, Unregistered Sales of Equity Securities, Financial Statements and Exhibits, Creation of a Direct Financial Obligation or an Obligation under an Off-Balance Sheet Arrangement of a Registrant

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K/A (Amendment No. 2) Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 March 8, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of

September 11, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 11, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOST

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 11, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation)

September 11, 2023 EX-99

EX-99

INVESTOR CONFERENCE Revolutionizing Cardiovascular Medicine With Epigenetics and AI S E P T E M B E R 2 0 2 3Certain statements and information included in this presentation constitute "forward-looking statements" within the meaning of the Private Securities Litigation Act of 1995.

September 11, 2023 EX-99.1

Cardio Diagnostics Holdings Inc. September 2023 Investor Presentation.

Exhibit 99.1

August 14, 2023 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended June 30, 2023 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Cardio Diagno

August 14, 2023 EX-10.1

Lease Agreement, dated July 20, 2023, between the Registrant and 246 Group LC dba North Point Crossing

Exhibit 10.1 246 GROUP, LC DBA NORTH POINT CROSSING CARDIO DIAGNOSTICS HOLDINGS, INC. COMMERCIAL LEASE-BUSINESS PROPERTY This Lease Agreement, executed in duplicate, made, and entered into this JULY 20TH, 2023 by and between 246 GROUP, LC., hereinafter referred to as LANDLORD, whose address for the purpose of this Lease is 52 STURGIS CORNER DRIVE, IOWA CITY, IA 52246, CARDIO DIAGNOSTICS HOLDINGS,

August 14, 2023 EX-10.2

Office Building Lease Agreement, dated June 15, 2023, between the Registrant and 311 W. Superior, L.L.C.

EXHIBIT 10.2 311 W. SUPERIOR CHICAGO, ILLINOIS OFFICE BUILDING LEASE TENANT: Cardio Diagnostics, Inc. TABLE OF CONTENTS Page 1. Lease of Premises 1 2. Definitions 1 3. Exhibits and Addenda 2 4. Lease Grant/Possession 3 5. Rent 3 6. Interest and Late Charges 7 7. Security Deposit 7 8. Option to Renew 8 9. Option to Relocate 8 10 Signage 8 11. Storage 9 12. Restoration 9 13. Parking 9 14. Tenant’s U

June 28, 2023 SC 13D/A

US14159C1036 / Cardio Diagnostics Holdings, Inc. / Dogan Meeshanthini Activist Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D Under the Securities Exchange Act of 1934 (Amendment No. 1) Cardio Diagnostics Holdings, Inc. (Name of Issuer) Common Stock, par value $0.00001 per share (Title of Class of Securities) 14159C103 (CUSIP Number) P. Rupert Russell, Esq. Shartsis Friese LLP One Maritime Plaza, 18th Floor San Francisco, CA 94111 Teleph

June 28, 2023 SC 13D/A

US14159C1036 / Cardio Diagnostics Holdings, Inc. / Philibert Robert Activist Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D Under the Securities Exchange Act of 1934 (Amendment No. 1) Cardio Diagnostics Holdings, Inc. (Name of Issuer) Common Stock, par value $0.00001 per share (Title of Class of Securities) 14159C103 (CUSIP Number) P. Rupert Russell, Esq. Shartsis Friese LLP One Maritime Plaza, 18th Floor San Francisco, CA 94111 Teleph

June 22, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 June 19, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS H

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 June 19, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Com

June 22, 2023 EX-10.1

Form of Board of Directors Agreement, dated June 19, 2023

EXHIBIT 10.1 BOARD OF DIRECTORS AGREEMENT This Board of Directors Agreement (“Agreement”) is made effective as, by and between Cardio Diagnostics Holdings, Inc., with its principal place of business at 400 N Aberdeen St, Suite 900, Chicago, IL 60642 (the “Company”) and , with an address at (“Director”), provides for director services, according to the following terms and conditions: I.Services Pro

June 5, 2023 8-K/A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K/A (Amendment No. 1) Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 March 8, 2023 Date of Report (Date of earliest event reported)

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K/A (Amendment No. 1) Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 March 8, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of

June 5, 2023 EX-10.1

Letter Agreement dated June 2, 2023 amending the Securities Purchase Agreement dated March 8, 2023 (which agreement was previously filed as Exhibit 10.1 to the Original 8-K on March 13, 2023)

Exhibit 10.1 June 2, 2023 Cardio Diagnostic Holdings, Inc. 400 North Aberdeen Street, Suite 900 Chicago, IL 60642 Ladies and Gentlemen: Reference is made to that certain Securities Purchase Agreement dated March 8, 2023 (the “SPA”), by and between YA II PN, Ltd. (“Yorkville”) and Cardio Diagnostics Holdings, Inc. (the “Company”). Capitalized terms used herein and not otherwise defined herein shall

May 30, 2023 EX-3.1

Third Amended and Restated Certificate of Incorporation of Cardio Diagnostics Holdings, Inc., dated May 30, 2023

Exhibit 3.1 THIRD AMENDED AND RESTATED CERTIFICATE OF INCORPORATION OF CARDIO DIAGNOSTICS HOLDINGS, INC. May 30, 2023 Cardio Diagnostics Holdings, Inc., a corporation existing under the laws of the State of Delaware (the “Corporation”), by its Chief Executive Officer, hereby certifies as follows: 1. The name of the Corporation is “Cardio Diagnostics Holdings, Inc.” 2. The Corporation’s Certificate

May 30, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 26, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HO

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 26, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Comm

May 16, 2023 EX-99.1

Cardio Diagnostics Holdings Inc. May 2023 Investor Presentation.

Exhibit 99.1

May 16, 2023 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 16, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HO

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 16, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Comm

May 16, 2023 EX-99.1

EX-99.1

COMPANY UPDATE Revolutionizing Cardiovascular Medicine With Epigenetics and AI M A Y 2 0 2 3Certain statements and information included in this presentation constitute "forward-looking statements" within the meaning of the Private Securities Litigation Act of 1995.

May 15, 2023 424B3

CARDIO DIAGNOSTICS HOLDINGS, INC. Primary Offering of 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Secondary Offering of 11,883,256 Shares of Common Stock 236,686 Warrants to Purchase Shares of Common Stock

Filed pursuant to Rule 424(b)(3) Registration No. 333-268759 PROSPECTUS SUPPLEMENT NO. 1 (to prospectus dated April 28, 2023) CARDIO DIAGNOSTICS HOLDINGS, INC. Primary Offering of 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Secondary Offering of 11,883,256 Shares of Common Stock 236,686 Warrants to Purchase Shares of Common Stock This prospectus supplement is being filed to

May 15, 2023 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended March 31, 2023 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Cardio Diagn

May 15, 2023 424B3

CARDIO DIAGNOSTICS HOLDINGS, INC. Up to 20,363,637 Shares of Common Stock

Filed pursuant to Rule 424(b)(3) Registration No. 333-271147 PROSPECTUS SUPPLEMENT NO. 1 (to prospectus dated April 11, 2023) CARDIO DIAGNOSTICS HOLDINGS, INC. Up to 20,363,637 Shares of Common Stock This prospectus supplement is being filed to update and supplement the information contained in the prospectus dated April 11, 2023 (the “Prospectus), related to the resale from time to time by the se

May 3, 2023 DEF 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☐ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☒ Definitive Proxy State

May 1, 2023 424B3

CARDIO DIAGNOSTICS HOLDINGS, INC. Primary Offering of 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Secondary Offering of 11,883,256 Shares of Common Stock 236,686 Warrants to Purchase Shares of Common Stock

Filed Pursuant to Rule 424(b)(3) File No. 333-268759 PROSPECTUS CARDIO DIAGNOSTICS HOLDINGS, INC. Primary Offering of 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Secondary Offering of 11,883,256 Shares of Common Stock 236,686 Warrants to Purchase Shares of Common Stock This prospectus relates to the issuance by us of up to an aggregate of 3,486,686 shares of our Common Stoc

April 24, 2023 POS AM

As filed with the U.S. Securities and Exchange Commission on April 23, 2023.

As filed with the U.S. Securities and Exchange Commission on April 23, 2023. Registration No. 333-268759 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 POST-EFFECTIVE AMENDMENT NO. 1 TO FORM S-1 POS AM REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 6770 87-0925574 (St

April 11, 2023 424B3

CARDIO DIAGNOSTICS HOLDINGS, INC. Up to 20,363,637 Shares of Common Stock

Filed Pursuant to Rule 424(b)(3) Registration No. 333-271147 PROSPECTUS CARDIO DIAGNOSTICS HOLDINGS, INC. Up to 20,363,637 Shares of Common Stock This prospectus relates to the resale from time to time by the selling securityholder named in this prospectus (the “Selling Stockholder”) of up to an aggregate of 20,363,637 shares of common stock, $0.00001 par value (the “Common Stock”) underlying thos

April 10, 2023 CORRESP

Cardio Diagnostics Holdings, Inc. 400 North Aberdeen Street, Suite 900 Chicago, IL 60642 April 10, 2023

Correspondence Cardio Diagnostics Holdings, Inc. 400 North Aberdeen Street, Suite 900 Chicago, IL 60642 April 10, 2023 VIA EDGAR Division of Corporation Finance Office of Life Sciences U.S. Securities & Exchange Commission 100 F Street, NE Washington, D.C. 20549 Attn: Tim Buchmiller Re: Cardio Diagnostics Holdings, Inc. Registration Statement on Form S-1 File No. 333-271147 Acceleration Request Re

April 7, 2023 PRE 14A

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 14A Proxy Statement Pursuant to Section 14(a) of the Securities Exchange Act of 1934 Filed by the Registrant ☒ Filed by a Party other than the Registrant ☐ Check the appropriate box: ☒ Preliminary Proxy Statement ☐ Confidential, for Use of the Commission Only (as permitted by Rule 14a-6(e)(2)) ☐ Definitive Proxy State

April 5, 2023 EX-FILING FEES

Filing Fee Table

EXHIBIT 107 Calculation of Filing Fee Tables Form S-1 (Form Type) Cardio Diagnostics Holdings, Inc.

April 5, 2023 S-1

As filed with the U.S. Securities and Exchange Commission on April 5, 2023.

As filed with the U.S. Securities and Exchange Commission on April 5, 2023. Registration No. 333- UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 6770 87-0925574 (State or other jurisdiction of incorporation or or

March 31, 2023 10-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2022 ☐ TRANSITION REPORT PURSUA

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2022 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File No. 001-41097 CARDIO DIAGNOSTICS HO

March 31, 2023 EX-4.5

Description of Securities

EXHIBIT 4.5 DESCRIPTION OF THE REGISTRANT’S SECURITIES REGISTERED PURSUANT TO SECTION 12 OF THE SECURITIES EXCHANGE ACT OF 1934 The following is a description of the capital stock of Cardio Diagnostics Holdings, Inc. (“Cardio,” the “Company,” “we,” “us,” and “our”) and certain provisions of our second amended and restated certificate of incorporation (the “certificate of incorporation”), our bylaw

March 31, 2023 EX-10.19

Amendment No. 1 to Engagement Letter, dated November 14, 2022, between the Registrant and The Benchmark Company, LLC

EXHIBIT 10.19 AMENDMENT NO. 1 ENGAGEMENT LETTER Amendment 1 dated November 14, 2022 (this “Amendment”) to the Engagement Letter dated as of May 13, 2022 between The Benchmark Company, LLC (“Benchmark”) and Cardio Diagnostics, Inc. [sic] (“Company”). WHEREAS, the parties entered into an engagement letter dated as of May 13, 2022 (the “Engagement Letter”) pursuant to which Benchmark agreed to act as

March 31, 2023 EX-10.18

Engagement Letter, dated as of May 13, 2022, between Mana Capital Acquisition Corp. and The Benchmark Company, LLC

EXHIBIT 10.18 [Logo] BENCHMARK May 13, 2022 STRICTLY CONFIDENTIAL Mana Capital Acquisition Corp. Jonathan Intrater Chief Executive Officer 8 The Green Suite 12490 Dover, DE 19901 Dear Mr. Intrater: This letter (the "Agreement") constitutes the agreement between Mana Capital Acquisition Corp. (the "Company") and The Benchmark Company, LLC ("Benchmark") that Benchmark shall serve as the exclusive fi

March 31, 2023 424B3

CARDIO DIAGNOSTICS HOLDINGS, INC. Primary Offering of 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Secondary Offering of 11,883,256 Shares of Common Stock 236,686 Warrants to Purchase Shares of Common Stock

Filed pursuant to Rule 424(b)(3)  Registration No. 333-268759 PROSPECTUS SUPPLEMENT NO. 1 (to prospectus dated January 24, 2023) CARDIO DIAGNOSTICS HOLDINGS, INC. Primary Offering of 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Secondary Offering of 11,883,256 Shares of Common Stock 236,686 Warrants to Purchase Shares of Common Stock This prospectus supplement is being filed

March 31, 2023 EX-21.1

List of Subsidiaries

EXHIBIT 21.1 Subsidiaries of Cardio Diagnostics Holdings, Inc. Cardio Diagnostics, Inc., a Delaware corporation

March 22, 2023 EX-FILING FEES

Filing Fee Table

EXHIBIT 107 Calculation of Filing Fee Tables Form S-8 (Form Type) Cardio Diagnostics Holdings, Inc.

March 22, 2023 S-8

As filed with the U.S. Securities and Exchange Commission on March 22, 2023.

As filed with the U.S. Securities and Exchange Commission on March 22, 2023. Registration No. 333- UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM S-8 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 6770 87-0925574 (State or other jurisdiction of incorporation or o

March 13, 2023 EX-99.1

Cardio Diagnostics Holdings, Inc Announces Financing Agreement with Yorkville Advisors Global, LP of up to $11.2 Million

EXHIBIT 99.1 Cardio Diagnostics Holdings, Inc Announces Financing Agreement with Yorkville Advisors Global, LP of up to $11.2 Million CHICAGO, IL March 8, 2023 – Cardio Diagnostics Holdings, Inc (the “Company”) (Nasdaq: CDIO), an artificial intelligence-powered precision cardiovascular medicine company, today announced that it has entered into a securities purchase agreement (“Securities Purchase

March 13, 2023 EX-10.3

Registration Rights Agreement, dated March 8, 2023, by and between the registrant and YA II PN, Ltd.

EXHIBIT 10.3 REGISTRATION RIGHTS AGREEMENT THIS REGISTRATION RIGHTS AGREEMENT (this “Agreement”), dated as of March 8, 2023 by and among CARDIO DIAGNOSTICS HOLDINGS, INC., a Delaware corporation (the “Company”), and YA II PN, Ltd., a Cayman Islands exempt limited partnership (the “Investor”). WITNESSETH WHEREAS: A.In connection with the Securities Purchase Agreement by and among the parties hereto

March 13, 2023 8-K

Regulation FD Disclosure, Entry into a Material Definitive Agreement, Unregistered Sales of Equity Securities, Financial Statements and Exhibits, Creation of a Direct Financial Obligation or an Obligation under an Off-Balance Sheet Arrangement of a Registrant, Other Events

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 March 8, 2023 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Com

March 13, 2023 EX-10.2

Form of Convertible Debenture

EXHIBIT 10.2 NEITHER THIS DEBENTURE NOR THE SECURITIES INTO WHICH THIS DEBENTURE IS CONVERTIBLE HAVE BEEN REGISTERED WITH THE SECURITIES AND EXCHANGE COMMISSION OR THE SECURITIES COMMISSION OF ANY STATE. THESE SECURITIES HAVE BEEN SOLD IN RELIANCE UPON AN EXEMPTION FROM REGISTRATION UNDER THE SECURITIES ACT OF 1933, AS AMENDED (THE “SECURITIES ACT”), AND, ACCORDINGLY, MAY NOT BE OFFERED OR SOLD EX

March 13, 2023 EX-10.1

Securities Purchase Agreement, dated March 8, 2023, by and between the registrant and YA II PN, Ltd.

EXHIBIT 10.1 SECURITIES PURCHASE AGREEMENT THIS SECURITIES PURCHASE AGREEMENT (this “Agreement”), dated as of March 8, 2023, is between CARDIO DIAGNOSTICS HOLDINGS, INC., a company incorporated under the laws of the State of Delaware, with principal executive offices located at 400 North Aberdeen Street, Suite 900, Chicago, IL 60642 (the “Company”), and each of the investors listed on the Schedule

March 13, 2023 EX-4.1

Convertible Debenture, dated March 8, 2023

EXHIBIT 4.1 NEITHER THIS DEBENTURE NOR THE SECURITIES INTO WHICH THIS DEBENTURE IS CONVERTIBLE HAVE BEEN REGISTERED WITH THE SECURITIES AND EXCHANGE COMMISSION OR THE SECURITIES COMMISSION OF ANY STATE. THESE SECURITIES HAVE BEEN SOLD IN RELIANCE UPON AN EXEMPTION FROM REGISTRATION UNDER THE SECURITIES ACT OF 1933, AS AMENDED (THE “SECURITIES ACT”), AND, ACCORDINGLY, MAY NOT BE OFFERED OR SOLD EXC

February 6, 2023 SC 13G/A

US14159C1036 / Cardio Diagnostics Holdings, Inc. / Weiss Asset Management LP Passive Investment

=============================================================================== UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.

January 25, 2023 424B3

CARDIO DIAGNOSTICS HOLDINGS, INC. Primary Offering of 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Secondary Offering of 11,883,256 Shares of Common Stock 236,686 Warrants to Purchase Shares of Common Stock

Filed Pursuant to Rule 424(b)(3) Registration No. 333-268759 PROSPECTUS CARDIO DIAGNOSTICS HOLDINGS, INC. Primary Offering of 3,486,686 Shares of Common Stock Issuable Upon Exercise of Warrants Secondary Offering of 11,883,256 Shares of Common Stock 236,686 Warrants to Purchase Shares of Common Stock This prospectus relates to the issuance by us of up to an aggregate of 3,486,686 shares of our Com

January 20, 2023 CORRESP

Cardio Diagnostics Holdings, Inc. 400 North Aberdeen Street, Suite 900 Chicago, IL 60642 January 20, 2023

Cardio Diagnostics Holdings, Inc. 400 North Aberdeen Street, Suite 900 Chicago, IL 60642 January 20, 2023 VIA EDGAR Division of Corporation Finance Office of Life Sciences U.S. Securities & Exchange Commission 100 F Street, NE Washington, D.C. 20549 Attn: Cindy Polynice Jason Drory Re: Cardio Diagnostics Holdings, Inc. Registration Statement on Form S-1 File No. 333-268759 Acceleration Request Req

January 13, 2023 CORRESP

P. Rupert Russell (415) 773-7243 January 13, 2023

P. Rupert Russell [email protected] (415) 773-7243 January 13, 2023 Via EDGAR U.S. Securities and Exchange Commission Division of Corporation Finance Office of Life Sciences 100 F Street, N.E. Washington, D.C. 20549 Attention: Cindy Polynice Jason Drory Re: Cardio Diagnostics Holdings, Inc. Amendment No. 1 to Registration Statement on Form S-1 Filed December 27, 2022 File No. 333-268759 Ladies an

January 13, 2023 S-1/A

As filed with the U.S. Securities and Exchange Commission on January 13, 2023.

As filed with the U.S. Securities and Exchange Commission on January 13, 2023. Registration No. 333-268759 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 AMENDMENT NO. 2 TO FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 6770 87-0925574 (State or other jurisdi

December 27, 2022 CORRESP

P. Rupert Russell (415) 773-7243 December 27, 2022

P. Rupert Russell [email protected] (415) 773-7243 December 27, 2022 Via EDGAR U.S. Securities and Exchange Commission Division of Corporation Finance Office of Life Sciences 100 F Street, N.E. Washington, D.C. 20549 Attention: Cindy Polynice Jason Drory Re: Cardio Diagnostics Holdings, Inc. Registration Statement on Form S-1 Filed December 12, 2022 File No. 333-268759 Ladies and Gentlemen: On be

December 27, 2022 S-1/A

As filed with the U.S. Securities and Exchange Commission on December 27, 2022.

As filed with the U.S. Securities and Exchange Commission on December 27, 2022. Registration No. 333-268759 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 AMENDMENT NO. 1 TO FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 6770 87-0925574 (State or other jurisd

December 12, 2022 EX-10.5

Form of Indemnification Agreement

Exhibit 10.5 INDEMNIFICATION AGREEMENT INDEMNIFICATION AGREEMENT (this ?Agreement?) effective as of , 202 by and between CARDIO DIAGNOSTICS HOLDINGS, INC., a Delaware corporation (the ?Company?) and (?Indemnitee?). R E C I T A L S A. The Company and Indemnitee recognize the continued difficulty in obtaining liability insurance for its directors, officers, employees, stockholders, controlling perso

December 12, 2022 EX-FILING FEES

Filing Fee Table

Exhibit 107 Calculation of Filing Fee Tables Form S-1 (Form Type) Cardio Diagnostics Holdings, Inc.

December 12, 2022 S-1

As filed with the U.S. Securities and Exchange Commission on December 12, 2022.

As filed with the U.S. Securities and Exchange Commission on December 12, 2022. Registration No. 333- UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM S-1 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 6770 87-0925574 (State or other jurisdiction of incorporation o

November 10, 2022 8-K

Financial Statements and Exhibits, Changes in Registrant's Certifying Accountant

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 November 9, 2022 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (

November 10, 2022 EX-16.1

Letter from MaloneBailey, LLP to the Securities and Exchange Commission, dated November 10, 2022

Exhibit 16.1 November 10, 2022 U.S. Securities and Exchange Commission 100 F Street, N.E. Washington, DC 20549 We have read the statements under Item 4.01 of the Current Report on Form 8-K of Cardio Diagnostics Holdings, Inc. to be filed with the Securities and Exchange Commission on or about November 10, 2022. We agree with all statements pertaining to us. We have no basis on which to agree or di

November 7, 2022 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ☒ QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended September 30, 2022 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Cardio D

November 4, 2022 SC 13D

CDIO / Cardio Diagnostics Holdings Inc / Philibert Robert Activist Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D Under the Securities Exchange Act of 1934 (Amendment No. ) Cardio Diagnostics Holdings Inc. (Name of Issuer) Common Stock, par value $0.00001 per share (Title of Class of Securities) 14159C103 (CUSIP Number) P. Rupert Russell, Esq. Shartsis Friese LLP One Maritime Plaza, 18th Floor San Francisco, CA 94111 Telephon

November 3, 2022 SC 13D

CDIO / Cardio Diagnostics Holdings Inc / Dogan Meeshanthini Activist Investment

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13D Under the Securities Exchange Act of 1934 (Amendment No. ) Cardio Diagnostics Holdings Inc. (Name of Issuer) Common Stock, par value $0.00001 per share (Title of Class of Securities) 14159C103 (CUSIP Number) P. Rupert Russell, Esq. Shartsis Friese LLP One Maritime Plaza, 18th Floor San Francisco, CA 94111 Telephon

October 31, 2022 EX-99.2

Cardio Diagnostics Holdings, Inc. to List on Nasdaq Following Successful Business Combination with Mana Capital Acquisition Corp. Cardio Diagnostics Holdings, Inc. Scheduled to Commence Trading on NASDAQ Under the Ticker Symbol “CDIO”

EX-99.2 7 ex99x2.htm EXHIBIT 99.2 PRESS RELEASE Exhibit 99.2 Cardio Diagnostics Holdings, Inc. to List on Nasdaq Following Successful Business Combination with Mana Capital Acquisition Corp. Cardio Diagnostics Holdings, Inc. Scheduled to Commence Trading on NASDAQ Under the Ticker Symbol “CDIO” CHICAGO, IL (October 26, 2022) – Cardio Diagnostics Holdings, Inc. ("Cardio" or the “Company”), a pionee

October 31, 2022 EX-21.1

List of Subsidiaries

Exhibit 21.1 Subsidiaries of Cardio Diagnostics Holdings, Inc. Cardio Diagnostics, Inc., a Delaware corporation

October 31, 2022 EX-99.1

UNAUDITED PRO FORMA CONDENSED COMBINED FINANCIAL INFORMATION

EXHIBIT 99.1 UNAUDITED PRO FORMA CONDENSED COMBINED FINANCIAL INFORMATION Capitalized terms used but not defined in this Exhibit 99.1 shall have the meanings ascribed to them in the Current Report on Form 8-K (the “Form 8-K”)filed with the Securities and Exchange Commission (the “SEC”) on October 31, 2022 and, if not defined in the Form 8-K, then in the final prospectus and definitive proxy statem

October 31, 2022 8-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 October 25, 2022 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTIC

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 October 25, 2022 Date of Report (Date of earliest event reported) CARDIO DIAGNOSTICS HOLDINGS, INC. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (

October 31, 2022 EX-2.3

Waiver Agreement dated as of October 25, 2022 with respect to Agreement and Plan of Merger dated as of May 27, 2022, as amended on September 15, 2022

Exhibit 2.3 WAIVER AGREEMENT This WAIVER AGREEMENT (the “Waiver Agreement”), dated as of October 25, 2022, is entered into by and by and among Cardio Diagnostics, Inc., a Delaware corporation (the “Company” or “Cardio”), Meeshanthini (Meesha) Dogan, as representative of the shareholders of the Company (the “Shareholders’ Representative”), Mana Capital Acquisition Corp., a Delaware corporation (“Pa

October 31, 2022 EX-3.1

Second Amended and Restated Certificate of Incorporation of Cardio Diagnostics Holdings, Inc., dated October 25, 2022

Exhibit 3.1 SECOND AMENDED AND RESTATED CERTIFICATE OF INCORPORATION OF MANA CAPITAL ACQUISITION CORP. October 25, 2022 Mana Capital Acquisition Corp., a corporation existing under the laws of the State of Delaware (the “Corporation”), by its Chief Executive Officer, hereby certifies as follows: 1. The name of the Corporation is “Mana Capital Acquisition Corp.” 2. The Corporation’s Certificate of

October 31, 2022 EX-10.4

Cardio Diagnostics Holdings, Inc. 2022 Equity Incentive Plan

Exhibit 10.4 CARDIO DIAGNOSTICS HOLDINGS INC. 2022 EQUITY INCENTIVE PLAN Table of Contents Page SECTION 1 Establishment and Purpose. 1 (a) Purpose 1 (b) Adoption and Term 1 SECTION 2 Definitions 1 SECTION 3 Administration 4 (a) Committee of the Board of Directors 5 (b) Authority. 5 (c) Exchange Program 5 (d) Delegation by the Committee 5 (e) Indemnification 6 SECTION 4 Eligibility and Award Limita

October 28, 2022 SC 13G/A

US56168P1049 / Mana Capital Acquisition Corp. / Feis Lawrence Michael - SCHEDULE 13-G/A Passive Investment

Schedule 13G OMB APPROVAL UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.

October 11, 2022 425

Mana Capital Acquisition Corp. Announces Effectiveness of Registration Statement and October 25, 2022 Special Meeting of Stockholders to Approve Business Combination with Cardio Diagnostics, Inc.

425 1 mana425.htm 425 Filed by Mana Capital Acquisition Corp. Pursuant to Rule 425 under the Securities Act of 1933 and deemed filed pursuant to Rule 14a-12 under the Securities Exchange Act of 1934 Subject Company: Mana Capital Acquisition Corp. Commission File No.: 001-41097 Mana Capital Acquisition Corp. Announces Effectiveness of Registration Statement and October 25, 2022 Special Meeting of S

October 7, 2022 424B3

PROXY STATEMENT FOR SPECIAL MEETING OF STOCKHOLDERS OF MANA CAPITAL ACQUISITION CORP. PROSPECTUS FOR SHARES OF COMMON STOCK OF MANA CAPITAL ACQUISITION CORP. Proxy Statement dated October 7, 2022 and first mailed to stockholders on or about October 7

Filed Pursuant to Rule 424(b)(3) Registration No. 333-265308 PROXY STATEMENT FOR SPECIAL MEETING OF STOCKHOLDERS OF MANA CAPITAL ACQUISITION CORP. PROSPECTUS FOR SHARES OF COMMON STOCK OF MANA CAPITAL ACQUISITION CORP. Proxy Statement dated October 7, 2022 and first mailed to stockholders on or about October 7, 2022 Dear Stockholders: You are cordially invited to attend the special meeting of the

October 5, 2022 S-4/A

Agreement and Plan of Merger dated as of May 27, 2022 by and among Mana Capital Acquisition Corp., Mana Merger Sub, Inc., Cardio Diagnostics, Inc., and Meeshanthini (Meesha) Dogan, as representatives of the shareholders (included as Annex A to the Proxy Statement/Prospectus)

As filed with the U.S. Securities and Exchange Commission on October 5, 2022 Registration No. 333-265308 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Amendment No. 5 to Form S-4 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 6770 87-09255674 (State or other jurisdictio

October 5, 2022 CORRESP

Mana Capital Acquisition Corp. 8 The Green, Suite 12490 Dover, DE 19901

Correspondence Mana Capital Acquisition Corp. 8 The Green, Suite 12490 Dover, DE 19901 October 5, 2022 VIA EDGAR Division of Corporation Finance Office of Life Sciences U.S. Securities & Exchange Commission 100 F Street, NE Washington, D.C. 20549 RE: Mana Capital Acquisition Corp. (the ?Company?) Registration Statement on Form S-4, as amended (File No. 333-265308) (the ?Registration Statement?) La

October 4, 2022 EX-10.5

Stock Escrow Agreement, dated November 22, 2021, by and among the Company, the Sponsor, Continental Stock Transfer & Trust Company, and the other holders party thereto.

EXHIBIT 10.5 STOCK ESCROW AGREEMENT STOCK ESCROW AGREEMENT, dated as of November 22, 2021 (?Agreement?), by and among MANA CAPITAL ACQUISITION CORP., a Delaware corporation (the ?Company?), MANA CAPITAL LLC, a Delaware limited liability company and a stockholder of the Company (?Sponsor?), JONATHAN INTRATER (?Intrater?), ALLAN LIU (?Liu?), LOREN MORTMAN (?Mortman?), and CONTINENTAL STOCK TRANSFER

October 4, 2022 EX-3.2

MANA Capital acqusition corp. (THE “CORPORATION”) (a Delaware corporation) ARTICLE I

EXHIBIT 3.2 BYLAWS OF MANA Capital acqusition corp. (THE ?CORPORATION?) (a Delaware corporation) ARTICLE I OFFICES Section 1.1. Registered Office. The registered office of the Corporation within the State of Delaware shall be located at either (a) the principal place of business of the Corporation in the State of Delaware or (b) the office of the corporation or individual acting as the Corporation

October 4, 2022 EX-10.2

Letter Agreement, dated November 22, 2021, by and between the Company and its chief executive officer.

EXHIBIT 10.2 November 22, 2021 Mana Capital Acquisition Corp. 8 The Green Suite #12490 Dover, DE 19901 Re: Initial Public Offering Ladies and Gentlemen: This letter (this ?Letter Agreement?) is being delivered to you in accordance with the Underwriting Agreement (the ?Underwriting Agreement?) entered into by and among Mana Capital Acquisition Corp., a Delaware corporation (the ?Company?), and Lade

October 4, 2022 EX-3.1

Amended and Restated Certificate of Incorporation.

EXHIBIT 3.1 AMENDED AND RESTATED CERTIFICATE OF INCORPORATION OF MANA CAPITAL ACQUISITION CORP. November 22, 2021 Mana Capital Acquisition Corp., a corporation existing under the laws of the State of Delaware (the ?Corporation?), by its Chief Executive Officer, hereby certifies as follows: 1. The name of the Corporation is ?Mana Capital Acquisition Corp.? 2. The Corporation?s Certificate of Incorp

October 4, 2022 EX-10.1

Letter Agreement, dated November 22, 2021, by and among the Company, its independent directors and the Sponsor.

EXHIBIT 10.1 November 22, 2021 Mana Capital Acquisition Corp. 8 The Green Suite #12490 Dover, DE 19901 Re: Initial Public Offering Ladies and Gentlemen: This letter (this ?Letter Agreement?) is being delivered to you in accordance with the Underwriting Agreement (the ?Underwriting Agreement?) entered into by and among Mana Capital Acquisition Corp., a Delaware corporation (the ?Company?), and Lade

October 4, 2022 EX-4.5

Rights Agreement, dated November 22, 2021, by and between the Company and Continental Stock Transfer & Trust Company, as rights agent.

EXHIBIT 4.5 RIGHTS AGREEMENT This Rights Agreement (this ?Agreement?) is made as of November 22, 2021 between Mana Capital Acquisition Corp., a Delaware corporation (the ?Company?), and Continental Stock Transfer & Trust Company, a New York corporation, with offices at 1 State Street, New York, New York 10004 (the ?Right Agent?). WHEREAS, the Company has received a firm commitment from Ladenburg T

October 4, 2022 EX-4.1

Specimen Unit Certificate.

EXHIBIT 4.1 SPECIMEN UNIT CERTIFICATE [] UNITS U-[?] SEE REVERSE FOR CERTAIN DEFINITIONS CUSIP [] MANA CAPITAL ACQUISITION CORP. UNITS CONSISTING OF ONE SHARE OF COMMON STOCK, A WARRANT TO PURCHASE ONE HALF OF ONE SHARE OF COMMON STOCK, AND ONE RIGHT TO RECEIVE ONE-SEVENTH OF ONE SHARE OF COMMON STOCK THIS CERTIFIES THAT is the owner of Units of Mana Capital Acquisition Corp., a Delaware corporati

October 4, 2022 EX-4.4

Warrant Agreement, dated November 22, 2021, by and between the Company and Continental Stock Transfer & Trust Company, as warrant agent.

EXHIBIT 4.4 MANA CAPITAL ACQUISITION CORP. WARRANT AGREEMENT THIS WARRANT AGREEMENT (this ?Agreement?), dated as of November 22, 2021, is by and between Mana Capital Acquisition Corp., a Delaware corporation (the ?Company?), and Continental Stock Transfer & Trust Company, a New York corporation with offices at 1 State Street, New York, New York 10004, as warrant agent (the ?Warrant Agent? or also

October 4, 2022 EX-1.2

Business Combination Marketing Agreement, dated November 22, 2021, by and among the Company, Ladenburg Thalmann, and I-Bankers Securities, Inc.

EXHIBIT 1.2 Ladenburg Thalmann & Co. Inc. 640 Fifth Avenue, 4th floor New York, New York 10019 November 22, 2021 Mana Capital Acquisition Corp. 8 The Green Suite #12490 Dover, Delaware 19901 Attention: Jonathan Intrater, Chief Executive Officer Ladies and Gentlemen: This is to confirm our agreement (this ?Agreement?) whereby Mana Capital Acquisition Corp, a Delaware corporation (?Company?), has re

October 4, 2022 EX-10.4

Registration Rights Agreement, dated November 22, 2021, by and among the Company, the Sponsor and the other holders party thereto.

EXHIBIT 10.4 REGISTRATION RIGHTS AGREEMENT THIS REGISTRATION RIGHTS AGREEMENT (this ?Agreement?) is entered into as of the 22nd day of November, 2021, by and among Mana Capital Acquisition Corp., a Delaware corporation (the ?Company?), and the undersigned parties listed under Investors on the signature page hereto (each, an ?Investor? and collectively, the ?Investors?). WHEREAS, the Investors and

October 4, 2022 EX-4.2

Specimen Common Stock Certificate.

EX-4.2 6 ex4x2.htm SPECIMEN COMMON STOCK CERTIFICATE. EXHIBIT 4.2 NUMBER SHARES SEE REVERSE FOR CERTAIN DEFINITIONS CUSIP MANA CAPITAL ACQUISITION CORP. A DELAWARE CORPORATION COMMON STOCK This Certifies that is the owner of FULLY PAID AND NON-ASSESSABLE SHARES OF COMMON STOCK, $0.00001 PAR VALUE PER SHARE EACH, OF MANA CAPITAL ACQUISITION CORP. (THE “CORPORATION”) transferable on the books of the

October 4, 2022 EX-10.3

Investment Management Trust Agreement, dated November 22, 2021, by and between the Company and Continental Stock Transfer & Trust Company, as trustee.

EX-10.3 11 ex10x3.htm INVESTMENT MANAGEMENT TRUST AGREEMENT, DATED NOVEMBER 22, 2021, BY AND BETWEEN THE COMPANY AND CONTINENTAL STOCK TRANSFER & TRUST COMPANY, AS TRUSTEE. EXHIBIT 10.3 INVESTMENT MANAGEMENT TRUST AGREEMENT This Investment Management Trust Agreement (this “Agreement”) is made effective as of November 22, 2021, by and between Mana Capital Acquisition Corp., a Delaware corporation (

October 4, 2022 S-4/A

Amendment dated September 15, 2022 to Agreement and Plan of Merger dated as of May 27, 2022 by and among Mana Capital Acquisition Corp., Mana Merger Sub, Inc., Cardio Diagnostics, Inc., and Meeshanthini (Meesha) Dogan, as representatives of the shareholders

As filed with the U.S. Securities and Exchange Commission on October 4, 2022 Registration No. 333-265308 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Amendment No. 4 to Form S-4/A REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 6770 87-09255674 (State or other jurisdict

October 3, 2022 CORRESP

****

Correspondence Michael A. Goldstein [email protected] Phone: (212) 440-5988 45 Broadway, 17th Floor New York, New York 10006 October 3, 2022 Via EDGAR Division of Corporation Finance Office of Life Sciences Securities and Exchange Commission Washington, D.C. 20549 Attn.: Ms. Doris Stacey Gama Mr. Joseph McCann Ms. Christine Torney Mr. Brian Cascio Re: Mana Capital Acquisition Corp Amend

September 23, 2022 EX-99.2

Mana Capital Acquisition Corp. Confirms Second Contribution to Trust Account to Extend Time Period to Complete a Business Combination

Exhibit 99.2 Mana Capital Acquisition Corp. Confirms Second Contribution to Trust Account to Extend Time Period to Complete a Business Combination Dover, DE, September 23, 2022?Mana Capital Acquisition Corp. (Nasdaq: MAAQU; MAAQ; MAAQW; MAAQR) (the ?Company? or ?Mana Capital?), a special purpose acquisition company (?Mana Capital?), announced today that for the purpose of consummating its previous

September 23, 2022 425

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 22, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL AC

425 1 mana425-092322.htm 425 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 22, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other juri

September 23, 2022 EX-10.1

Form of Promissory Note

EX-10.1 2 ex10x1.htm UNSECURED PROMISSORY NOTE Exhibit 10.1 THIS PROMISSORY NOTE (“NOTE”) HAS NOT BEEN REGISTERED UNDER THE SECURITIES ACT OF 1933, AS AMENDED (THE “SECURITIES ACT”). THIS NOTE HAS BEEN ACQUIRED FOR INVESTMENT ONLY AND MAY NOT BE SOLD, TRANSFERRED OR ASSIGNED IN THE ABSENCE OF REGISTRATION OF THE RESALE THEREOF UNDER THE SECURITIES ACT OR AN OPINION OF COUNSEL REASONABLY SATISFACTO

September 23, 2022 EX-10.1

Form of Promissory Note

EX-10.1 2 ex10x1.htm UNSECURED PROMISSORY NOTE Exhibit 10.1 THIS PROMISSORY NOTE (“NOTE”) HAS NOT BEEN REGISTERED UNDER THE SECURITIES ACT OF 1933, AS AMENDED (THE “SECURITIES ACT”). THIS NOTE HAS BEEN ACQUIRED FOR INVESTMENT ONLY AND MAY NOT BE SOLD, TRANSFERRED OR ASSIGNED IN THE ABSENCE OF REGISTRATION OF THE RESALE THEREOF UNDER THE SECURITIES ACT OR AN OPINION OF COUNSEL REASONABLY SATISFACTO

September 23, 2022 EX-99.1

Mana Capital Acquisition Corp. Announces Intention to Further Extend Period to Consummate Initial Business Combination

Exhibit 99.1 Mana Capital Acquisition Corp. Announces Intention to Further Extend Period to Consummate Initial Business Combination Dover, DE, September 22, 2022?Mana Capital Acquisition Corp. (Nasdaq: MAAQU; MAAQ; MAAQW; MAAQR) (the ?Company? or ?Mana Capital?) today announced that its Board of Directors has approved a second extension of the period of time available to the Company to consummate

September 23, 2022 EX-99.2

Mana Capital Acquisition Corp. Confirms Second Contribution to Trust Account to Extend Time Period to Complete a Business Combination

Exhibit 99.2 Mana Capital Acquisition Corp. Confirms Second Contribution to Trust Account to Extend Time Period to Complete a Business Combination Dover, DE, September 23, 2022?Mana Capital Acquisition Corp. (Nasdaq: MAAQU; MAAQ; MAAQW; MAAQR) (the ?Company? or ?Mana Capital?), a special purpose acquisition company (?Mana Capital?), announced today that for the purpose of consummating its previous

September 23, 2022 8-K

Financial Statements and Exhibits, Creation of a Direct Financial Obligation or an Obligation under an Off-Balance Sheet Arrangement of a Registrant, Other Events

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 22, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (C

September 23, 2022 EX-99.1

Mana Capital Acquisition Corp. Announces Intention to Further Extend Period to Consummate Initial Business Combination

Exhibit 99.1 Mana Capital Acquisition Corp. Announces Intention to Further Extend Period to Consummate Initial Business Combination Dover, DE, September 22, 2022?Mana Capital Acquisition Corp. (Nasdaq: MAAQU; MAAQ; MAAQW; MAAQR) (the ?Company? or ?Mana Capital?) today announced that its Board of Directors has approved a second extension of the period of time available to the Company to consummate

September 21, 2022 EX-99.1

Cardio Diagnostics Announces Issuance of U.S. Patent for Compositions and Methods for Detecting Predisposition to Cardiovascular Disease

Exhibit 99.1 Cardio Diagnostics Announces Issuance of U.S. Patent for Compositions and Methods for Detecting Predisposition to Cardiovascular Disease CHICAGO, Ill ? Cardio Diagnostics, Inc. ("Cardio Diagnostics" or the "Company"), a pioneering precision cardiovascular testing company, today announced that the U.S. Patent and Trademark Office has issued to the University of Iowa Research Foundation

September 21, 2022 8-K

Regulation FD Disclosure, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 21, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (C

September 21, 2022 425

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 21, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL AC

425 1 mana425-092022.htm 425 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 21, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other juri

September 21, 2022 EX-99.1

Cardio Diagnostics Announces Issuance of U.S. Patent for Compositions and Methods for Detecting Predisposition to Cardiovascular Disease

Exhibit 99.1 Cardio Diagnostics Announces Issuance of U.S. Patent for Compositions and Methods for Detecting Predisposition to Cardiovascular Disease CHICAGO, Ill – Cardio Diagnostics, Inc. ("Cardio Diagnostics" or the "Company"), a pioneering precision cardiovascular testing company, today announced that the U.S. Patent and Trademark Office has issued to the University of Iowa Research Foundation

September 15, 2022 EX-2.1

Amendment dated September 15, 2022 to Agreement and Plan of Merger dated as of May 27, 2022 by and among Mana Capital Acquisition Corp., Mana Merger Sub, Inc., Cardio Diagnostics, Inc., and Meeshanthini (Meesha) Dogan, as representative of the shareholders

Exhibit 2.1 FIRST AMENDMENT TO MERGER AGREEMENT AND PLAN OF REORGANIZATION This FIRST AMENDMENT TO MERGER AGREEMENT AND PLAN OF REORGANIZATION (the ?Amendment?) is made and entered into as of September 15, 2022 by and among Cardio Diagnostics, Inc., a Delaware corporation (the ?Company? or ?Cardio?), Meeshanthini (Meesha) Dogan, as representative of the shareholders of the Company (the ?Shareholde

September 15, 2022 S-4/A

Amendment dated September 15, 2022 to Agreement and Plan of Merger dated as of May 27, 2022 by and among Mana Capital Acquisition Corp., Mana Merger Sub, Inc., Cardio Diagnostics, Inc., and Meeshanthini (Meesha) Dogan, as representatives of the stockholders. (included as Annex A to the proxy statement/prospectus filed as part of this registration statement).

As filed with the U.S. Securities and Exchange Commission on September 15, 2022 Registration No. 333-265308 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Amendment No. 3 to Form S-4 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 6770 87-09255674 (State or other jurisdic

September 15, 2022 CORRESP

****

Correspondence Michael A. Goldstein [email protected] Phone: (212) 440-5988 45 Broadway, 17th Floor New York, New York 10006 September 15, 2022 Via EDGAR Division of Corporation Finance Office of Life Sciences Securities and Exchange Commission Washington, D.C. 20549 Attn.: Ms. Doris Stacey Gama Mr. Joseph McCann Ms. Christine Torney Mr. Brian Cascio Re: Mana Capital Acquisition Corp Am

September 15, 2022 425

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 15, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL AC

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 15, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (C

September 15, 2022 EX-FILING FEES

Filing Fee Table.

Exhibit 107 Calculation of Filing Fee Tables Form S-4 (Form Type) Mana Capital Acquisition Corp.

September 15, 2022 8-K

Entry into a Material Definitive Agreement, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 September 15, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (C

September 15, 2022 EX-2.1

Amendment dated September 15, 2022 to Agreement and Plan of Merger dated as of May 27, 2022 by and among Mana Capital Acquisition Corp., Mana Merger Sub, Inc., Cardio Diagnostics, Inc., and Meeshanthini (Meesha) Dogan, as representatives of the shareholders

Exhibit 2.1 FIRST AMENDMENT TO MERGER AGREEMENT AND PLAN OF REORGANIZATION This FIRST AMENDMENT TO MERGER AGREEMENT AND PLAN OF REORGANIZATION (the ?Amendment?) is made and entered into as of September 15, 2022 by and among Cardio Diagnostics, Inc., a Delaware corporation (the ?Company? or ?Cardio?), Meeshanthini (Meesha) Dogan, as representative of the shareholders of the Company (the ?Shareholde

September 15, 2022 EX-10.19

First Amendment to Exclusive License Agreement between Cardio Diagnostics, Inc. and the University of Iowa Research Foundation dated September 2, 2022

Exhibit 10.19 FIRST AMENDMENT TO EXCLUSIVE LICENSE AGREEMENT This FIRST AMENDMENT TO EXCLUSIVE LICENSE AGREEMENT (the ?First Amendment?) is made and entered into as of September 2, 2022, by and between the University of Iowa Research Foundation, a nonprofit corporation organized and existing under the laws of the State of Iowa and having an address at 200 South Capitol Street, 2660 University Capi

August 26, 2022 EX-99.1

EX-99.1

August 26, 2022 EX-99.1

Transforming Cardiovascular Cardiovascular Health Through Our AI - Driven Integrated Genetic - Epigenetic Epigenetic Engine Œ INVESTOR PRESENTATION August 2022 MANA CAPITAL Acquisition Corp. Disclaimer General This presentation is for informational p

Exhibit 99.1 Transforming Cardiovascular Cardiovascular Health Through Our AI - Driven Integrated Genetic - Epigenetic Epigenetic Engine Œ INVESTOR PRESENTATION August 2022 MANA CAPITAL Acquisition Corp. Disclaimer General This presentation is for informational purposes and only to assist in evaluating the proposed business combination (the “Busi nes s Combination”) between Mana Capital Acquisitio

August 26, 2022 EX-99.1

EX-99.1

August 26, 2022 8-K

Regulation FD Disclosure, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 August 26, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Comm

August 26, 2022 EX-99.1

Transforming Cardiovascular Cardiovascular Health Through Our AI - Driven Integrated Genetic - Epigenetic Epigenetic Engine Œ INVESTOR PRESENTATION August 2022 MANA CAPITAL Acquisition Corp. Disclaimer General This presentation is for informational p

Exhibit 99.1 Transforming Cardiovascular Cardiovascular Health Through Our AI - Driven Integrated Genetic - Epigenetic Epigenetic Engine Œ INVESTOR PRESENTATION August 2022 MANA CAPITAL Acquisition Corp. Disclaimer General This presentation is for informational purposes and only to assist in evaluating the proposed business combination (the “Busi nes s Combination”) between Mana Capital Acquisitio

August 26, 2022 425

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 August 26, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUI

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 August 26, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Comm

August 25, 2022 EX-10.1

Form of Promissory Note

Exhibit 10.1 THIS PROMISSORY NOTE (“NOTE”) HAS NOT BEEN REGISTERED UNDER THE SECURITIES ACT OF 1933, AS AMENDED (THE “SECURITIES ACT”). THIS NOTE HAS BEEN ACQUIRED FOR INVESTMENT ONLY AND MAY NOT BE SOLD, TRANSFERRED OR ASSIGNED IN THE ABSENCE OF REGISTRATION OF THE RESALE THEREOF UNDER THE SECURITIES ACT OR AN OPINION OF COUNSEL REASONABLY SATISFACTORY IN FORM, SCOPE AND SUBSTANCE TO THE COMPANY

August 25, 2022 EX-99.1

Mana Capital Acquisition Corp. Announces Intention to Extend Period to Consummate Initial Business Combination

Exhibit 99.1 Mana Capital Acquisition Corp. Announces Intention to Extend Period to Consummate Initial Business Combination Dover, DE, August 23, 2022—Mana Capital Acquisition Corp. (Nasdaq: MAAQU; MAAQ; MAAQW; MAAQR) (the “Company” or “Mana Capital”) today announced that its Board of Directors has approved an extension of the period of time available to the Company to consummate its initial busin

August 25, 2022 EX-99.1

Mana Capital Acquisition Corp. Announces Intention to Extend Period to Consummate Initial Business Combination

Exhibit 99.1 Mana Capital Acquisition Corp. Announces Intention to Extend Period to Consummate Initial Business Combination Dover, DE, August 23, 2022—Mana Capital Acquisition Corp. (Nasdaq: MAAQU; MAAQ; MAAQW; MAAQR) (the “Company” or “Mana Capital”) today announced that its Board of Directors has approved an extension of the period of time available to the Company to consummate its initial busin

August 25, 2022 EX-99.2

Mana Capital Acquisition Corp. Confirms Contribution to Trust Account to Extend Time Period to Complete a Business Combination

Exhibit 99.2 Mana Capital Acquisition Corp. Confirms Contribution to Trust Account to Extend Time Period to Complete a Business Combination Dover, DE, August 25, 2022?Mana Capital Acquisition Corp. (Nasdaq: MAAQU; MAAQ; MAAQW; MAAQR) (the ?Company? or ?Mana Capital?), a special purpose acquisition company (?Mana Capital?), announced today that for the purpose of consummating its previously announc

August 25, 2022 EX-99.2

Mana Capital Acquisition Corp. Confirms Contribution to Trust Account to Extend Time Period to Complete a Business Combination

Exhibit 99.2 Mana Capital Acquisition Corp. Confirms Contribution to Trust Account to Extend Time Period to Complete a Business Combination Dover, DE, August 25, 2022?Mana Capital Acquisition Corp. (Nasdaq: MAAQU; MAAQ; MAAQW; MAAQR) (the ?Company? or ?Mana Capital?), a special purpose acquisition company (?Mana Capital?), announced today that for the purpose of consummating its previously announc

August 25, 2022 EX-10.1

Form of Promissory Note

Exhibit 10.1 THIS PROMISSORY NOTE (“NOTE”) HAS NOT BEEN REGISTERED UNDER THE SECURITIES ACT OF 1933, AS AMENDED (THE “SECURITIES ACT”). THIS NOTE HAS BEEN ACQUIRED FOR INVESTMENT ONLY AND MAY NOT BE SOLD, TRANSFERRED OR ASSIGNED IN THE ABSENCE OF REGISTRATION OF THE RESALE THEREOF UNDER THE SECURITIES ACT OR AN OPINION OF COUNSEL REASONABLY SATISFACTORY IN FORM, SCOPE AND SUBSTANCE TO THE COMPANY

August 25, 2022 425

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 August 23, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUI

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 August 23, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Comm

August 25, 2022 8-K

Financial Statements and Exhibits, Creation of a Direct Financial Obligation or an Obligation under an Off-Balance Sheet Arrangement of a Registrant, Other Events

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 August 23, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Comm

August 23, 2022 EX-10.16

Employment Agreement, executed as of May 27, 2022, between Cardio Diagnostics, Inc. and Timur Dogan

Exhibit 10.16 EMPLOYMENT AGREEMENT THIS EMPLOYMENT AGREEMENT (this “Agreement”) is made and entered into as of May 27, 2022, by and between Cardio Diagnostics, Inc., a Delaware corporation (the “Company”), and Timur Dogan (the “Executive” and together with the Company referred to as the “Parties”). For good and valuable consideration, the receipt and sufficiency of which are hereby acknowledged, t

August 23, 2022 EX-10.15

Employment Agreement, executed as of May 27, 2022, between Cardio Diagnostics, Inc. and Elisa Luqman

Exhibit 10.15 EMPLOYMENT AGREEMENT THIS EMPLOYMENT AGREEMENT (this ?Agreement?) is made and entered into as of May 27, 2022, by and between Cardio Diagnostics, Inc., a Delaware corporation (the ?Company?), and Elisa Luqman (the ?Executive? and together with the Company referred to as the ?Parties?). For good and valuable consideration, the receipt and sufficiency of which are hereby acknowledged,

August 23, 2022 S-4/A

As filed with the U.S. Securities and Exchange Commission on August 23, 2022

As filed with the U.S. Securities and Exchange Commission on August 23, 2022 Registration No. 333-265308 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Amendment No. 2 to Form S-4 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 6770 87-09255674 (State or other jurisdictio

August 23, 2022 CORRESP

****

Correspondence Michael A. Goldstein [email protected] Phone: (212) 440-5988 45 Broadway, 17th Floor New York, New York 10006 August 23, 2022 Via EDGAR Division of Corporation Finance Office of Life Sciences Securities and Exchange Commission Washington, D.C. 20549 Attn.: Ms. Doris Stacey Gama Mr. Joseph McCann Ms. Christine Torney Mr. Brian Cascio Re: Mana Capital Acquisition Corp Amend

August 23, 2022 EX-10.17

Employment Agreement, executed as of May 18, 2022, between Cardio Diagnostics, Inc. and Khullani Abdullahi

EX-10.17 9 ex10x17.htm EMPLOYMENT AGREEMENT- CARDIO DIAGNOSTICS, INC. AND KHULLANI ABDULLAHI Exhibit 10.17 EMPLOYMENT AGREEMENT THIS EMPLOYMENT AGREEMENT (this “Agreement”) is made and entered into as of May 19, 2022, by and between Cardio Diagnostics, Inc., a Delaware corporation (the “Company”), and Khullani Abdullahi (the “Employee” and together with the Company referred to as the “Parties”) to

August 23, 2022 EX-10.14

Employment Agreement, executed as of May 27, 2022, between Cardio Diagnostics, Inc. and Robert Philibert

Exhibit 10.14 EMPLOYMENT AGREEMENT THIS EMPLOYMENT AGREEMENT (this ?Agreement?) is made and entered into as of May 27, 2022, by and between Cardio Diagnostics, Inc., a Delaware corporation (the ?Company?), and Robert Philibert, (the ?Executive? and together with the Company referred to as the ?Parties?). For good and valuable consideration, the receipt and sufficiency of which are hereby acknowled

August 23, 2022 EX-10.18

Non-Executive Chairman and Consulting Agreement between Cardio Diagnostics, Inc. and Warren Hosseinion

Exhibit 10.18 NON-EXECUTIVE CHAIRMAN AND CONSULTING AGREEMENT This NON-EXECUTIVE CHAIRMAN AND CONSULTING AGREEMENT (this “Agreement”), dated as of May 27, 2022 is made and entered into by and between Cardio Diagnostics Inc. (the “Company”) and Warren Hosseinion (the “Consultant”). Where appropriate Company and Consultant will each be referred to as a “Party,” or collectively as the “Parties. WITNE

August 23, 2022 EX-10.11

Exclusive License Agreement between Cardio Diagnostics, LLC and the University of Iowa Research Foundation dated May 2, 2017

EX-10.11 4 ex10x11.htm EXHIBIT 10.11 EXCLUSIVE LICENSE AGREEMENT BETWEEN CARDIO DIAGNOSTICS, LLC AND THE UNIVERSITY OF IOWA RESEARCH FOUNDATION DATED MAY 2, 2017. Exhibit 10.11 Exclusive License Agreement This license agreement ("Agreement") effective as of this 2nd day of May, 2017 ("Effective Date") is by and between the University of Iowa Research Foundation, a nonprofit corporation organized a

August 23, 2022 EX-10.7

Form of Indemnification Escrow Agreement.

Exhibit 10.7 INDEMNIFICATION ESCROW AGREEMENT This INDEMNIFICATION ESCROW AGREEMENT (the “Agreement”), dated as of [·], 2022 by and among Continental Stock Transfer & Trust Company, as escrow agent (the “Escrow Agent”), Mana Capital Acquisition Corp. (the “Parent”) as Indemnified Party and as representative of all Indemnified Parties, and Meeshanthini (Meesha) Dogan (the “Stockholders’ Representat

August 23, 2022 EX-10.13

Employment Agreement, executed as of May 27, 2022, between Cardio Diagnostics, Inc. and Meeshanthini Dogan

Exhibit 10.13 EMPLOYMENT AGREEMENT THIS EMPLOYMENT AGREEMENT (this “Agreement”) is made and entered into as of May 27, 2022, by and between Cardio Diagnostics, Inc., a Delaware corporation (the “Company”), and Meeshanthini Dogan, (the “Executive” and together with the Company referred to as the “Parties”). For good and valuable consideration, the receipt and sufficiency of which are hereby acknowl

August 10, 2022 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ? QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended June 30, 2022 ? TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Mana Capital

July 27, 2022 EX-10.11

Exclusive License Agreement between Cardio Diagnostics, LLC and the University of Iowa Research Foundation dated May 2, 2017.

Exhibit 10.11 Certain identified information has been excluded from this exhibit because it is both not material and is the type that the registrant treats as private or confidential. Information that was omitted has been noted in this document with a placeholder identified by the mark “[***]”. Exclusive License Agreement This license agreement ("Agreement") effective as of this 2nd day of May, 20

July 27, 2022 EX-10.12

Service Agreement

EX-10.12 3 ex10x12.htm EXHIBIT 10.12 SERVICE AGREEMENT DATED MAY 11, 2021 BETWEEN MANA CAPITAL, LLC AND JONATHAN INTRATER. Exhibit 10.12 Service Agreement Mana Capital LLC, a Delaware limited liability company ("Sponsor") hereby offers to Jonathan Intrater ("Executive") the following terms for his service in terms of working as a director and officer of Mana Acquisition. Corp. ("Company", hereinaf

July 27, 2022 AW

Mana Capital Acquisition Corp. 8 The Green, Suite 12490 Dover, Delaware 19901

Mana Capital Acquisition Corp. 8 The Green, Suite 12490 Dover, Delaware 19901 July 26, 2022 VIA EDGAR Securities and Exchange Commission Division of Corporation Finance Office of Life Science 100 F St., N.E. Washington, D.C. 20549 Attn: Ms. Doris Stacey Gama Mr. Joseph McCann Ms. Christine Torney Mr. Brian Cascio Re: Mana Capital Acquisition Corp. Request for Withdrawal on Form AW of Amendment to

July 27, 2022 S-4/A

As filed with the U.S. Securities and Exchange Commission on July 27, 2022

As filed with the U.S. Securities and Exchange Commission on July 27, 2022 Registration No. 333-265308 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Amendment No. 1 to Form S-4 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 6770 87-09255674 (State or other jurisdiction

July 27, 2022 EX-99.6

Consent of The Benchmark Company, LLC

EX-99.6 6 ex99x6.htm EXHIBIT 99.6 Exhibit 99.6 CONSENT OF THE BENCHMARK COMPANY, LLC July 25, 2022 Mana Capital Acquisition Corp. 8 The Green, Suite 12490 Dover, DE 19901 Attn: Board of Directors RE: Proxy Statement / Prospectus of Mana Capital Acquisition Corp. (“Mana”) which forms part of the Registration Statement on Form S-4 of Mana (the “Registration Statement”). Dear Members of the Board of

July 25, 2022 CORRESP

****

Correspondence Michael A. Goldstein [email protected] Phone: (212) 440-5988 45 Broadway, 17th Floor New York, New York 10006 July 25, 2022 Via EDGAR Division of Corporation Finance Office of Life Sciences Securities and Exchange Commission Washington, D.C. 20549 Attn.: Ms. Doris Stacey Gama Mr. Joseph McCann Ms. Christine Torney Mr. Brian Cascio Re: Mana Capital Acquisition Corp Registr

July 25, 2022 EX-10.12

Service Agreement dated May 11, 2021 between Mana Capital, LLC and Jonathan Intrater.

Exhibit 10.12 Service Agreement Mana Capital LLC, a Delaware limited liability company ("Sponsor") hereby offers to Jonathan Intrater ("Executive") the following terms for his service in terms of working as a director and officer of Mana Acquisition. Corp. ("Company", hereinafter, Sponsor, Executive and Company arc referred to as a "Party" in singular or "Parties" in plural), a Delaware corporatio

July 25, 2022 S-4/A

As filed with the U.S. Securities and Exchange Commission on July 25, 2022

S-4/A 1 manas4a1.htm FORM S-4/A1 As filed with the U.S. Securities and Exchange Commission on July 25, 2022 Registration No. 333-265308 UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Amendment No. 1 to Form S-4 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 6770 87-09255

July 25, 2022 EX-99.6

Consent of The Benchmark Company, LLC.

EX-99.6 6 ex99x6.htm EXHIBIT 99.6 Exhibit 99.6 CONSENT OF THE BENCHMARK COMPANY, LLC July 25, 2022 Mana Capital Acquisition Corp. 8 The Green, Suite 12490 Dover, DE 19901 Attn: Board of Directors RE: Proxy Statement / Prospectus of Mana Capital Acquisition Corp. (“Mana”) which forms part of the Registration Statement on Form S-4 of Mana (the “Registration Statement”). Dear Members of the Board of

July 25, 2022 EX-10.11

Exclusive License Agreement between Cardio Diagnostics, LLC and the University of Iowa Research Foundation dated May 2, 2017.

Exhibit 10.11 Certain identified information has been excluded from this exhibit because it is both not material and is the type that the registrant treats as private or confidential. Information that was omitted has been noted in this document with a placeholder identified by the mark “[***]”. Exclusive License Agreement This license agreement ("Agreement") effective as of this 2nd day of May, 20

May 31, 2022 EX-99.1

Mana Capital Acquisition Corp. and Cardio Diagnostics, Inc. Announce Definitive Business Combination Agreement to Create Publicly Listed Company Set to Transform Cardiovascular Disease Through Epigenetics

Exhibit 99.1 Mana Capital Acquisition Corp. and Cardio Diagnostics, Inc. Announce Definitive Business Combination Agreement to Create Publicly Listed Company Set to Transform Cardiovascular Disease Through Epigenetics ? Cardio Diagnostics, Inc. has pioneered a proprietary artificial intelligence-driven Integrated Genetic-Epigenetic Engine? that enables improved diagnosis and prevention of cardiova

May 31, 2022 8-K

Regulation FD Disclosure, Entry into a Material Definitive Agreement, Financial Statements and Exhibits

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 27, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Commiss

May 31, 2022 EX-10.2

Form of Non-Compete Agreement

EX-10.2 4 ex10x2.htm NON-COMPETITION AND NON-SOLICITATION AGREEMENT Exhibit 10.2 NON-COMPETITION AND NON-SOLICITATION AGREEMENT This Non-Competition and Non-Solicitation Agreement (this “Agreement”) is entered into as of [●], 2022 by and between Mana Capital Acquisition Corp., a Delaware corporation (“Purchaser”), and [●] (the “Executive”, and together with Purchaser, the “Parties”, and each a “Pa

May 31, 2022 EX-99.1

Preliminary Proxy Card

Exhibit 99.1 PROXY THIS PROXY WILL BE VOTED AS DIRECTED. IF NO DIRECTIONS ARE GIVEN, THIS PROXY WILL BE VOTED ??FOR?? PROPOSALS 1 THROUGH 6 BELOW. YOUR VOTE IS IMPORTANT. PLEASE VOTE TODAY. SPECIAL MEETING OF STOCKHOLDERS , 2022 This Proxy is Solicited On Behalf Of The Board Of Directors Vote Your Proxy by mail: Mark, sign and date your proxy card and return it in the postage-paid envelope provide

May 31, 2022 EX-99.1

Mana Capital Acquisition Corp. and Cardio Diagnostics, Inc. Announce Definitive Business Combination Agreement to Create Publicly Listed Company Set to Transform Cardiovascular Disease Through Epigenetics

Exhibit 99.1 Mana Capital Acquisition Corp. and Cardio Diagnostics, Inc. Announce Definitive Business Combination Agreement to Create Publicly Listed Company Set to Transform Cardiovascular Disease Through Epigenetics ● Cardio Diagnostics, Inc. has pioneered a proprietary artificial intelligence-driven Integrated Genetic-Epigenetic Engine™ that enables improved diagnosis and prevention of cardiova

May 31, 2022 S-4

Power of Attorney (Included on the signature page of the initial filing of this Registration Statement).

As filed with the U.S. Securities and Exchange Commission on May 31, 2022 Registration No. 333- UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form S-4 REGISTRATION STATEMENT UNDER THE SECURITIES ACT OF 1933 MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 6770 87-09255674 (State or other jurisdiction of incorporation or organi

May 31, 2022 EX-10.8

Form of Non-Competition and Non-Solicitation Agreement

EX-10.8 3 ex10x8.htm NON-COMPETITION AND NON-SOLICITATION AGREEMENT Exhibit 10.8 NON-COMPETITION AND NON-SOLICITATION AGREEMENT This Non-Competition and Non-Solicitation Agreement (this “Agreement”) is entered into as of [●], 2022 by and between Mana Capital Acquisition Corp., a Delaware corporation (“Purchaser”), and [●] (the “Executive”, and together with Purchaser, the “Parties”, and each a “Pa

May 31, 2022 EX-99.2

Consent of James Intrater to be named as a director.

EX-99.2 7 ex99x2.htm CONSENT TO BE NAMED AS A DIRECTOR Exhibit 99.2 Consent to be Named as a Director In connection with the filing by Mana Capital Acquisition Corp. (the “Company”) of the Registration Statement on Form S-4 (the “Registration Statement”) with the Securities and Exchange Commission under the Securities Act of 1933, as amended (the “Securities Act”), I hereby consent, pursuant to Ru

May 31, 2022 EX-99.4

Consent of Oded Levy to be named as a director.

Exhibit 99.4 Consent to be Named as a Director In connection with the filing by Mana Capital Acquisition Corp. (the ?Company?) of the Registration Statement on Form S-4 (the ?Registration Statement?) with the Securities and Exchange Commission under the Securities Act of 1933, as amended (the ?Securities Act?), I hereby consent, pursuant to Rule 438 of the Securities Act, to being named in the Reg

May 31, 2022 EX-FILING FEES

Filing Fee Table

EX-FILING FEES 11 ex107.htm CALCULATION OF FILING FEE TABLES Exhibit 107 Calculation of Filing Fee Tables Form S-4 (Form Type) Mana Capital Acquisition Corp. (Exact Name of Registrant as Specified in its Charter) CALCULATION OF REGISTRATION FEE Security Type Security Class Title Fee Calculation or Carry Forward Rule Amount Registered Proposed Maximum Offering Price Per Security Maximum Aggregate O

May 31, 2022 EX-10.6

Form of Lock-up Agreement

EX-10.6 2 ex10x6.htm LOCK-UP AGREEMENT Exhibit 10.6 LOCK-UP AGREEMENT THIS LOCK-UP AGREEMENT (this “Agreement”) is dated as of [·], 2022, by and between the stockholder of Cardio Diagnostics, Inc. set forth on the signature page to this Agreement (the “Holder”) and Mana Capital Acquisition Corp., a Delaware corporation (the “Parent”). Capitalized terms used and not otherwise defined herein shall h

May 31, 2022 EX-2.1

Merger Agreement and Plan of Reorganization by and among Cardio Diagnostics, Inc., Meeshanthini (Meesha) Dogan, as Representative of the Shareholders of the Company, Mana Capital Acquisition Corp. and Mana Merger Sub, Inc., dated May 27, 2022*

Exhibit 2.1 MERGER AGREEMENT AND PLAN OF REORGANIZATION BY AND AMONG CARDIO DIAGNOSTICS, INC., MEESHANTHINI (MEESHA) DOGAN, AS REPRESENTATIVE OF THE SHAREHOLDERS OF THE COMPANY, MANA CAPITAL ACQUISITION CORP. AND MANA MERGER SUB INC. DATED AS OF MAY 27, 2022 TABLE OF CONTENTS Page ARTICLE I DEFINITIONS 1 1.1 Definitions 1 1.2 Construction 16 ARTICLE II AGREEMENT AND PLAN OF MERGER 17 2.1 The Merge

May 31, 2022 EX-99.3

Consent of Stanley Lau to be named as a director.

Exhibit 99.3 Consent to be Named as a Director In connection with the filing by Mana Capital Acquisition Corp. (the “Company”) of the Registration Statement on Form S-4 (the “Registration Statement”) with the Securities and Exchange Commission under the Securities Act of 1933, as amended (the “Securities Act”), I hereby consent, pursuant to Rule 438 of the Securities Act, to being named in the Reg

May 31, 2022 EX-2.1

Agreement and Plan of Merger dated as of May 27, 2022 by and among Mana Capital Acquisition Corp., Mana Merger Sub, Inc., Cardio Diagnostics, Inc., and Meeshanthini (Meesha) Dogan, as representatives of the shareholders (included as Annex A to the Proxy Statement/Prospectus)

Exhibit 2.1 MERGER AGREEMENT AND PLAN OF REORGANIZATION BY AND AMONG CARDIO DIAGNOSTICS, INC., MEESHANTHINI (MEESHA) DOGAN, AS REPRESENTATIVE OF THE SHAREHOLDERS OF THE COMPANY, MANA CAPITAL ACQUISITION CORP. AND MANA MERGER SUB INC. DATED AS OF MAY 27, 2022 TABLE OF CONTENTS Page ARTICLE I DEFINITIONS 1 1.1 Definitions 1 1.2 Construction 16 ARTICLE II AGREEMENT AND PLAN OF MERGER 17 2.1 The Merge

May 31, 2022 EX-10.1

Form of Lock-up Agreement

Exhibit 10.1 LOCK-UP AGREEMENT THIS LOCK-UP AGREEMENT (this “Agreement”) is dated as of [·], 2022, by and between the stockholder of Cardio Diagnostics, Inc. set forth on the signature page to this Agreement (the “Holder”) and Mana Capital Acquisition Corp., a Delaware corporation (the “Parent”). Capitalized terms used and not otherwise defined herein shall have the meanings given such terms in th

May 31, 2022 425

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 27, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISIT

425 1 mana425.htm UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 May 27, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incor

May 31, 2022 EX-99.5

Consent of Brandon Sim to be named as a director.

EX-99.5 10 ex99x5.htm CONSENT TO BE NAMED AS A DIRECTOR Exhibit 99.5 Consent to be Named as a Director In connection with the filing by Mana Capital Acquisition Corp. (the “Company”) of the Registration Statement on Form S-4 (the “Registration Statement”) with the Securities and Exchange Commission under the Securities Act of 1933, as amended (the “Securities Act”), I hereby consent, pursuant to R

May 31, 2022 EX-10.2

Form of Non-Compete Agreement

EX-10.2 4 ex10x2.htm NON-COMPETITION AND NON-SOLICITATION AGREEMENT Exhibit 10.2 NON-COMPETITION AND NON-SOLICITATION AGREEMENT This Non-Competition and Non-Solicitation Agreement (this “Agreement”) is entered into as of [●], 2022 by and between Mana Capital Acquisition Corp., a Delaware corporation (“Purchaser”), and [●] (the “Executive”, and together with Purchaser, the “Parties”, and each a “Pa

May 31, 2022 EX-10.1

Form of Lock-up Agreement

Exhibit 10.1 LOCK-UP AGREEMENT THIS LOCK-UP AGREEMENT (this ?Agreement?) is dated as of [?], 2022, by and between the stockholder of Cardio Diagnostics, Inc. set forth on the signature page to this Agreement (the ?Holder?) and Mana Capital Acquisition Corp., a Delaware corporation (the ?Parent?). Capitalized terms used and not otherwise defined herein shall have the meanings given such terms in th

May 12, 2022 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-Q (Mark One) ? QUARTERLY REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the quarterly period ended March 31, 2022 ? TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File Number: 001-41097 Mana Capital

March 31, 2022 10-K

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2021 ☐ TRANSITION REPORT PURSUA

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 FORM 10-K (Mark One) ☒ ANNUAL REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the fiscal year ended December 31, 2021 ☐ TRANSITION REPORT PURSUANT TO SECTION 13 OR 15(d) OF THE SECURITIES EXCHANGE ACT OF 1934 For the transition period from to Commission File No. 001-41097 MANA CAPITAL ACQUISIT

March 31, 2022 EX-4.6

Description of Securities *

Exhibit 4.6 DESCRIPTION OF THE REGISTRANT?S SECURITIES REGISTERED PURSUANT TO SECTION 12 OF THE SECURITIES EXCHANGE ACT OF 1934 As of the date of its Annual Report on Form 10-K for the fiscal year ended December 31, 2022, Mana Capital Acquisition Corp. has four classes of securities registered under Section 12 of the Securities Exchange Act of 1934, as amended (the ?Exchange Act?): (1) our units;

February 11, 2022 SC 13G

Weiss Asset Management LP

=============================================================================== UNITED STATES SECURITIES AND EXCHANGE COMMISSION WASHINGTON, D.

January 25, 2022 8-K

Financial Statements and Exhibits, Changes in Registrant's Certifying Accountant

8-K 1 mana8k.htm FORM 8-K UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 January 19, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdict

January 25, 2022 EX-16.1

Letter to Securities and Exchange Commission from Marcum Bernstein & Pinchuk, LLP dated January 25, 2022

EX-16.1 2 ex16x1.htm EXHIBIT 16.1 Exhibit 16.1 New York Office 7 Penn Plaza, Suite 830 New York, NY 10001 T 212-379-7900 January 25, 2022 Securities and Exchange Commission 100 F Street, NE Washington, DC 20549 Commissioners: We have read the statements made by Mana Capital Acquisition Corp. under Item 4.01 of its Form 8-K dated January 19, 2022. We agree with the statements concerning our Firm in

January 12, 2022 8-K

Financial Statements and Exhibits, Other Events

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 January 12, 2022 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Com

January 12, 2022 EX-99.1

Mana Capital Acquisition Corp. Announces the Separate Trading of its Common Stock, Warrants and Rights, Commencing January 14, 2022

EX-99.1 2 ex99x1.htm EXHIBIT 99.1 Exhibit 99.1 For Immediate Release Mana Capital Acquisition Corp. Announces the Separate Trading of its Common Stock, Warrants and Rights, Commencing January 14, 2022 Dover, DE – January 12, 2022 – Mana Capital Acquisition Corp. (Nasdaq: MAAQU) (“Mana” or the “Company”), today announced that, commencing January 14, 2022, holders of the 6,500,000 units sold in the

January 11, 2022 SC 13G/A

Feis Lawrence Michael - SCHEDULE 13G/A

OMB APPROVAL UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 OMB Number:3235-0145 Expires:Febuary 28, 2009 Estimated average burden hours per response ....10.4 SCHEDULE 13G Under the Securities and Exchange Act of 1934 (Amendment No. 1 )* Mana Capital Acquisition Corp. (Name of Issuer) Common stock, par value $ 0.00001 (Title of Class of Securities) 56168P203 (CUSIP Number)

December 6, 2021 EX-99.1

MANA CAPITAL ACQUISITION CORP. BALANCE SHEET

Exhibit 99.1 MANA CAPITAL ACQUISITION CORP. BALANCE SHEET Actual as of November 26, 2021 Pro Forma Adjustments As Adjusted (Unaudited) (Unaudited) ASSETS Current Assets Cash $ 887,872 $ (60,000 ) $ 827,872 Prepaid Expenses - short term 199,725 ? 199,725 Total Current Assets 1,087,597 (60,000 ) 1,027,597 Prepaid Expenses - long term 89,755 ? 89,755 Cash Held in Trust 62,000,000 3,000,000 65,000,000

December 6, 2021 8-K

Unregistered Sales of Equity Securities, Financial Statements and Exhibits, Other Events

8-K 1 mana8k.htm FORM 8-K UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 November 30, 2021 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdic

December 3, 2021 SC 13D

Mana Capital LLC - SCHEDULE 13D

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington 20549 SCHEDULE 13D Under the Securities Exchange Act of 1934 (Amendment No.

December 3, 2021 SC 13G

Saba Capital Management, L.P. - FORM SC 13G

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13G Under the Securities Exchange Act of 1934 (Amendment No. )* Mana Capital Acquisition Corp (Name of Issuer) Common Stock, $0.0001 par value (Title of Class of Securities) 56168P203 (CUSIP Number) November 23, 2021 (Date of Event which Requires Filing of this Statement) Check the appropriate box to designate the rul

December 3, 2021 EX-99.1

INDEX TO FINANCIAL STATEMENT

EX-99.1 2 manaex99z1.htm PRESS RELEASE EXHIBIT 99.1 INDEX TO FINANCIAL STATEMENT Page Audited Financial Statement of Mana Capital Acquisition Corp.: Report of Independent Registered Public Accounting Firm F-2 Balance Sheet as of November 26, 2021 F-3 Notes to Financial Statement F-4 F-1 REPORT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM To the Shareholders and Board of Directors of Mana Capit

December 3, 2021 8-K

Unregistered Sales of Equity Securities, Financial Statements and Exhibits, Other Events

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 Form 8-K Current Report Pursuant to Section 13 or 15(d) of the Securities Exchange Act of 1934 November 26, 2021 Date of Report (Date of earliest event reported) MANA CAPITAL ACQUISITION CORP. (Exact name of registrant as specified in its charter) Delaware 001-41097 87-0925574 (State or other jurisdiction of incorporation) (Co

December 2, 2021 SC 13G

Feis Lawrence Michael - SCHEDULE 13G

Schedule 13G OMB APPROVAL UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.

November 30, 2021 SC 13G

Space Summit Capital LLC - SC 13G

UNITED STATES SECURITIES AND EXCHANGE COMMISSION Washington, D.C. 20549 SCHEDULE 13G Under the Securities Exchange Act of 1934 (Amendment No. )* MANA CAPITAL ACQUISITION CORP. (Name of Issuer) Units (Title of Class of Securities) 56168P203 (CUSIP Number) November 23, 2021 (Date of Event which Requires Filing of this Statement) Check the appropriate box to designate the rule pursuant to which this

November 26, 2021 EX-1.2

Business Combination Marketing Agreement, dated November 22, 2021, by and among the Company, Ladenburg Thalmann, and I-Bankers Securities, Inc.

EXHIBIT 1.2 Ladenburg Thalmann & Co. Inc. 640 Fifth Avenue, 4th floor New York, New York 10019 November 22, 2021 Mana Capital Acquisition Corp. 8 The Green Suite #12490 Dover, Delaware 19901 Attention: Jonathan Intrater, Chief Executive Officer Ladies and Gentlemen: This is to confirm our agreement (this ?Agreement?) whereby Mana Capital Acquisition Corp, a Delaware corporation (?Company?), has re

November 26, 2021 EX-10.1

Letter Agreement, dated November 22, 2021, by and among the Company, its independent directors and the Sponsor.

EXHIBIT 10.1 November 22, 2021 Mana Capital Acquisition Corp. 8 The Green Suite #12490 Dover, DE 19901 Re: Initial Public Offering Ladies and Gentlemen: This letter (this ?Letter Agreement?) is being delivered to you in accordance with the Underwriting Agreement (the ?Underwriting Agreement?) entered into by and among Mana Capital Acquisition Corp., a Delaware corporation (the ?Company?), and Lade

November 26, 2021 EX-99.2

Mana Capital Acquisition Corp. Announces Closing of $62 Million Initial Public Offering

EX-99.2 14 manaex99z2.htm PRESS RELEASE EXHIBIT 99.2 For Immediate Release Mana Capital Acquisition Corp. Announces Closing of $62 Million Initial Public Offering Dover, DE – November 26, 2021 – Mana Capital Acquisition Corp. (Nasdaq: MAAQU) (“Mana” or the “Company”), a newly incorporated blank check company, today announced the closing of its initial public offering. The offering was priced at $1

Fintel data has been cited in the following publications:
Daily Mail Fox Business Business Insider Wall Street Journal The Washington Post Bloomberg Financial Times Globe and Mail
NASDAQ.com Reuters The Guardian Associated Press FactCheck.org Snopes Politifact
Federal Register The Intercept Forbes Fortune Magazine TheStreet Time Magazine Canadian Broadcasting Corporation International Business Times
Cambridge University Press Investopedia MarketWatch NY Daily News Entrepreneur Newsweek Barron's El Economista